8UUM
Prototypic SARS-CoV-2 spike (containing K417) in the open conformation
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2.7 |
Energy Filter | GIF Bioquantum |
Detector | FEI FALCON III (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.9 |
Number of Particle | 1004968 |
Software | cryoSPARC,PHENIX,Latitude,Coot |
Symmetry | C1 |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |