8UTK

IL-23R minibinder - 23R-B04dslf02IB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-19
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97918
Spacegroup name	P 31 2 1
Unit cell lengths	55.414, 55.414, 85.834
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.990 - 1.940
R-factor	0.2205
Rwork	0.218
R-free	0.26730
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.772
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.990	2.009
High resolution limit [Å]	1.940	1.940
Rmerge	0.511	2.587
Rpim	0.116	0.583
Number of reflections	11624	1121
<I/σ(I)>	3.62	0.77
Completeness [%]	98.8	98.16
Redundancy	20	20.4
CC(1/2)	0.979	0.372

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	293	0.2 M Lithium sulfate 0.1 M Sodium acetate pH 4.5 and 50% (v/v) PEG 400.