8UTA
yjdF riboswitch from R. gauvreauii in complex with proflavine bound to Fab BL3-6 S97N
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-07-20 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 1 |
Unit cell lengths | 63.873, 75.138, 95.727 |
Unit cell angles | 87.25, 87.99, 87.72 |
Refinement procedure
Resolution | 63.790 - 3.050 |
R-factor | 0.2272 |
Rwork | 0.224 |
R-free | 0.26910 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.191 |
Data reduction software | MOSFLM |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 95.560 | 3.210 |
High resolution limit [Å] | 3.050 | 3.050 |
Rmerge | 0.094 | 2.559 |
Rmeas | 0.106 | 2.910 |
Rpim | 0.050 | 1.364 |
Number of reflections | 32504 | 4517 |
<I/σ(I)> | 8.4 | 0.5 |
Completeness [%] | 95.7 | |
Redundancy | 4.5 | |
CC(1/2) | 0.998 | 0.364 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 0.1 M HEPES pH 7.5, 0.2 M ammonium acetate, 25% PEG 3350 |