8URL
The Apo NanJ structure from Clostridium perfringens
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-002 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-01-20 |
Detector | DECTRIS PILATUS 200K |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 66.226, 48.804, 73.324 |
Unit cell angles | 90.00, 93.95, 90.00 |
Refinement procedure
Resolution | 22.890 - 2.200 |
R-factor | 0.2032 |
Rwork | 0.201 |
R-free | 0.24850 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.873 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (3.7) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.000 | 2.300 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 23654 | 1095 |
<I/σ(I)> | 12.5 | |
Completeness [%] | 99.9 | |
Redundancy | 3.1 | |
CC(1/2) | 0.992 | 0.909 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1M HEPES pH 7.5 2.0M (NH4)2SO4 |