8UQS
Crystal structure of the Opossum p53 tetramerization domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE 7B2 |
Synchrotron site | CHESS |
Beamline | 7B2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-10-19 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 38.275, 50.887, 60.240 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.310 - 1.350 |
R-factor | 0.1716 |
Rwork | 0.169 |
R-free | 0.20180 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.069 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.310 | 1.398 |
High resolution limit [Å] | 1.350 | 1.350 |
Number of reflections | 24100 | 1080 |
<I/σ(I)> | 12.46 | |
Completeness [%] | 90.5 | |
Redundancy | 5.6 | |
CC(1/2) | 0.999 | 0.662 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 296 | 0.1 M Tris pH 8.5, 20% PEG 1000 |