8UQD

Crystal structure of RNF168 (RING)-UbcH5c fused to H2A-H2B via a 20-residue linker (condition 2. RING not modeled in density)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2018-11-28
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 32 2 1
Unit cell lengths	108.903, 108.903, 86.076
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.020 - 3.893
R-factor	0.2972
Rwork	0.295
R-free	0.32170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5egg
RMSD bond length	0.002
RMSD bond angle	0.467
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.970
High resolution limit [Å]	3.893	3.893
Rmerge	0.109	0.393
Rmeas	0.121	0.434
Rpim	0.049	0.176
Number of reflections	5587	544
<I/σ(I)>	11.47	3.26
Completeness [%]	98.3	99.27
Redundancy	5.1	5
CC(1/2)	0.997	0.952

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	288	Crystals were obtained by mixing 2 microliters of the protein fusion (12 mg/mL) in 10 mM HEPES, pH 7.5, 600 mM NaCl, 1 mM TCEP and 2 microliters of the reservoir solution containing 4% tacsimate, pH 6, 13.5% PEG3350