8UPI
Structure of a periplasmic peptide binding protein from Mesorhizobium sp. AP09 bound to aminoserine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | Agilent SuperNova |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-07-06 |
Detector | RIGAKU HyPix-6000HE |
Wavelength(s) | 1.54184 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.247, 58.920, 139.355 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.510 - 1.550 |
R-factor | 0.1815 |
Rwork | 0.179 |
R-free | 0.22850 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.801 |
Data reduction software | CrysalisPro |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.510 | 1.580 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmerge | 0.078 | 0.503 |
Rmeas | 0.089 | 0.634 |
Rpim | 0.041 | 0.378 |
Total number of observations | 254272 | 9261 |
Number of reflections | 67507 | 3194 |
<I/σ(I)> | 7.8 | 2.1 |
Completeness [%] | 99.2 | |
Redundancy | 3.8 | 2.9 |
CC(1/2) | 0.985 | 0.445 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 30% PEG 4000 |