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8UP4

Crystal structure of Lsd18 (a flavin-dependent monooxygenase)

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 17-ID
Synchrotron siteAPS
Beamline17-ID
Temperature [K]100
Detector technologyPIXEL
Collection date2017-08-12
DetectorDECTRIS EIGER2 X 9M
Wavelength(s)1.000000
Spacegroup nameP 1
Unit cell lengths46.354, 61.790, 75.355
Unit cell angles74.64, 81.70, 77.11
Refinement procedure
Resolution36.749 - 1.510
R-factor0.142
Rwork0.140
R-free0.17850
Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.009
RMSD bond angle1.022
Data reduction softwareXDS
Data scaling softwareAimless
Phasing softwarePHASER
Refinement softwarePHENIX ((1.16_3549: ???))
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]58.4501.590
High resolution limit [Å]1.5101.510
Rmerge0.0580.416
Rmeas0.0690.496
Rpim0.0360.266
Number of reflections11763216922
<I/σ(I)>11.62.9
Completeness [%]95.994.1
Redundancy3.53.4
CC(1/2)0.9980.871
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP291.150.1 M Tris pH8.5, 0.85 M NaCl, 32% (w/v) PEG4000

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