8UP4
Crystal structure of Lsd18 (a flavin-dependent monooxygenase)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-08-12 |
Detector | DECTRIS EIGER2 X 9M |
Wavelength(s) | 1.000000 |
Spacegroup name | P 1 |
Unit cell lengths | 46.354, 61.790, 75.355 |
Unit cell angles | 74.64, 81.70, 77.11 |
Refinement procedure
Resolution | 36.749 - 1.510 |
R-factor | 0.142 |
Rwork | 0.140 |
R-free | 0.17850 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.022 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.16_3549: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.450 | 1.590 |
High resolution limit [Å] | 1.510 | 1.510 |
Rmerge | 0.058 | 0.416 |
Rmeas | 0.069 | 0.496 |
Rpim | 0.036 | 0.266 |
Number of reflections | 117632 | 16922 |
<I/σ(I)> | 11.6 | 2.9 |
Completeness [%] | 95.9 | 94.1 |
Redundancy | 3.5 | 3.4 |
CC(1/2) | 0.998 | 0.871 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 0.1 M Tris pH8.5, 0.85 M NaCl, 32% (w/v) PEG4000 |