8ULC

LSD1-CoREST in complex with T15, long soaking

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-02
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.96546
Spacegroup name	I 2 2 2
Unit cell lengths	118.324, 179.606, 234.349
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.540 - 2.820
R-factor	0.232
Rwork	0.231
R-free	0.25520
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.467
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.610	2.870
High resolution limit [Å]	2.800	2.800
Rmerge	0.095	1.421
Rmeas	0.106	1.622
Rpim	0.047	0.755
Total number of observations	271458	18312
Number of reflections	60042	4396
<I/σ(I)>	11.4	1.2
Completeness [%]	97.9
Redundancy	4.5	4.2
CC(1/2)	0.996	0.315

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1.2 M Sodium Potassium Tartrate, 100 mM ADA pH 6.5