8UKU

RNA polymerase II elongation complex with Fapy-dG lesion with CMP added

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-16
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.97648
Spacegroup name	C 1 2 1
Unit cell lengths	166.103, 224.009, 192.541
Unit cell angles	90.00, 99.98, 90.00

Refinement procedure

Resolution	49.240 - 3.600
R-factor	0.2588
Rwork	0.258
R-free	0.30150
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.779
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.240	3.570
High resolution limit [Å]	3.510	3.510
Rmerge	0.389	4.735
Rmeas	0.420	5.140
Rpim	0.158	1.971
Total number of observations	599066	30258
Number of reflections	85895	4532
<I/σ(I)>	6.1	0.5
Completeness [%]	99.4
Redundancy	7	6.7
CC(1/2)	0.991	0.215

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	390 mM ammonium phosphate (pH 6.0), 5 mM DTT, 5 mM dioxane, and 9-13% (w/v) PEG 6,000