8UKU
RNA polymerase II elongation complex with Fapy-dG lesion with CMP added
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.3 |
Synchrotron site | ALS |
Beamline | 5.0.3 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-06-16 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.97648 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 166.103, 224.009, 192.541 |
Unit cell angles | 90.00, 99.98, 90.00 |
Refinement procedure
Resolution | 49.240 - 3.600 |
R-factor | 0.2588 |
Rwork | 0.258 |
R-free | 0.30150 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.779 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.240 | 3.570 |
High resolution limit [Å] | 3.510 | 3.510 |
Rmerge | 0.389 | 4.735 |
Rmeas | 0.420 | 5.140 |
Rpim | 0.158 | 1.971 |
Total number of observations | 599066 | 30258 |
Number of reflections | 85895 | 4532 |
<I/σ(I)> | 6.1 | 0.5 |
Completeness [%] | 99.4 | |
Redundancy | 7 | 6.7 |
CC(1/2) | 0.991 | 0.215 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 390 mM ammonium phosphate (pH 6.0), 5 mM DTT, 5 mM dioxane, and 9-13% (w/v) PEG 6,000 |