8UKT
RNA polymerase II elongation complex with Fapy-dG lesion with AMP added
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-02-25 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.9774 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 156.681, 221.393, 190.539 |
Unit cell angles | 90.00, 97.67, 90.00 |
Refinement procedure
Resolution | 47.750 - 3.600 |
R-factor | 0.2522 |
Rwork | 0.251 |
R-free | 0.28950 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.686 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.750 | 3.650 |
High resolution limit [Å] | 3.580 | 3.580 |
Rmerge | 0.581 | 3.668 |
Rmeas | 0.627 | 3.974 |
Rpim | 0.236 | 1.519 |
Total number of observations | 528303 | 30534 |
Number of reflections | 75622 | 4498 |
<I/σ(I)> | 4.8 | 0.6 |
Completeness [%] | 100.0 | |
Redundancy | 7 | 6.8 |
CC(1/2) | 0.966 | 0.218 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 390 mM ammonium phosphate (pH 6.0), 5 mM DTT, 5 mM dioxane, and 9-13% (w/v) PEG 6,000 |