8UKS
RNA polymerase II elongation complex with Fapy-dG lesion soaking with CTP before chemistry
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-03-23 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.9774 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 157.118, 221.626, 189.961 |
| Unit cell angles | 90.00, 97.40, 90.00 |
Refinement procedure
| Resolution | 47.760 - 3.400 |
| R-factor | 0.2675 |
| Rwork | 0.267 |
| R-free | 0.30150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.831 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.760 | 3.450 |
| High resolution limit [Å] | 3.390 | 3.390 |
| Rmerge | 0.524 | 4.497 |
| Rmeas | 0.566 | 4.874 |
| Rpim | 0.214 | 1.858 |
| Total number of observations | 615254 | 30346 |
| Number of reflections | 89064 | 4492 |
| <I/σ(I)> | 4.6 | 0.6 |
| Completeness [%] | 99.9 | |
| Redundancy | 6.9 | 6.8 |
| CC(1/2) | 0.984 | 0.350 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 390 mM ammonium phosphate (pH 6.0), 5 mM DTT, 5 mM dioxane, and 9-13% (w/v) PEG 6,000 |
