8UKR

RNA polymerase II elongation complex with Fapy-dG lesion soaking with ATP before chemistry

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-27
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.9765
Spacegroup name	C 1 2 1
Unit cell lengths	159.617, 222.632, 190.697
Unit cell angles	90.00, 97.69, 90.00

Refinement procedure

Resolution	47.960 - 3.780
R-factor	0.2623
Rwork	0.261
R-free	0.30980
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.898
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.960	3.660
High resolution limit [Å]	3.590	3.590
Rmerge	0.574	4.892
Rmeas	0.620	2.161
Rpim	0.232	2.161
Number of reflections	76483	4424
<I/σ(I)>	4.5	0.4
Completeness [%]	99.4
Redundancy	7
CC(1/2)	0.985	0.464

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	390 mM ammonium phosphate (pH 6.0), 5 mM DTT, 5 mM dioxane, and 9-13% (w/v) PEG 6,000