8UKQ
RNA polymerase II elongation complex with Fapy-dG lesion in apo state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-03-23 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 161.540, 223.485, 191.472 |
| Unit cell angles | 90.00, 98.38, 90.00 |
Refinement procedure
| Resolution | 48.440 - 3.500 |
| R-factor | 0.2686 |
| Rwork | 0.268 |
| R-free | 0.30240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.726 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.440 | 3.540 |
| High resolution limit [Å] | 3.480 | 3.480 |
| Rmerge | 0.552 | 3.710 |
| Rmeas | 0.597 | 4.036 |
| Rpim | 0.226 | 1.573 |
| Total number of observations | 594677 | 30052 |
| Number of reflections | 85915 | 4596 |
| <I/σ(I)> | 4.7 | 0.6 |
| Completeness [%] | 99.9 | |
| Redundancy | 6.9 | 6.5 |
| CC(1/2) | 0.981 | 0.199 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 390 mM ammonium phosphate (pH 6.0), 5 mM DTT, 5 mM dioxane, and 9-13 % (w/v) PEG 6,000 |
