8UKO

cAMP-dependent protein kinase A catalytic domain in complex with voltage gated calcium channel peptide ternary complex 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.033
Spacegroup name	I 41
Unit cell lengths	118.036, 118.036, 58.008
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.560 - 2.890
R-factor	0.2132
Rwork	0.210
R-free	0.25930
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.432
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.20rc1_4392)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.000	35.000	2.960
High resolution limit [Å]	2.890	7.110	2.890
Rmerge	0.058	0.023	1.124
Rmeas	0.065	0.026	1.271
Rpim	0.030	0.012	0.583
Number of reflections	9121	636	615
<I/σ(I)>	7.8
Completeness [%]	99.9	99.2	100
Redundancy	4.7	4.6	4.6
CC(1/2)		0.999	0.342

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	277.15	0.1 M Succinic Acid pH 7.0, 15% PEG 3350