8UKI
Crystal structure of 04_A06 Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-03 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.680, 133.280, 43.360 |
| Unit cell angles | 90.00, 111.26, 90.00 |
Refinement procedure
| Resolution | 38.700 - 1.750 |
| Rwork | 0.170 |
| R-free | 0.20500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ngb |
| Data reduction software | iMOSFLM (v7.4) |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (v1.20.1-4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.700 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.120 | 0.710 |
| Rpim | 0.080 | 0.450 |
| Number of reflections | 52273 | 2575 |
| <I/σ(I)> | 7.7 | 1.8 |
| Completeness [%] | 97.0 | 96 |
| Redundancy | 6.6 | 6.1 |
| CC(1/2) | 0.990 | 0.820 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 8% v/v Tacsimate, pH 7.0, 20% w/v PEG3350 |
