8UK7

Periplasmic domain of Escherichia coli CpxA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 12.3.1
Synchrotron site	ALS
Beamline	12.3.1
Temperature [K]	110
Detector technology	CCD
Collection date	2013-08-01
Detector	ADSC QUANTUM 315
Wavelength(s)	1.03316
Spacegroup name	P 21 21 21
Unit cell lengths	37.160, 43.163, 186.981
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.190 - 1.800
R-factor	0.2062
Rwork	0.204
R-free	0.24650
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.150
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.200	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.070	1.090
Rmeas	0.076	1.160
Rpim	0.028	0.408
Number of reflections	28337	2769
<I/σ(I)>	13.75	2.68
Completeness [%]	98.1	96.6
Redundancy	8	7.9
CC(1/2)	0.997	0.958

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	294.15	100 mM Tris-HCl pH 7.5, 350 mM NaCl, 20% v/v 2-methyl-2,4-pentanediol