8UK4
Crystal structure of human polymerase eta with incoming dTMPnPP nucleotide opposite urea lesion
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2023-02-07 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.97872 |
Spacegroup name | P 61 |
Unit cell lengths | 98.795, 98.795, 80.290 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.780 - 3.020 |
R-factor | 0.1788 |
Rwork | 0.174 |
R-free | 0.26480 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.985 |
Data reduction software | HKL-2000 (v721.2) |
Data scaling software | HKL-2000 (v721.2) |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.070 |
High resolution limit [Å] | 3.020 | 3.020 |
Rpim | 0.040 | 0.300 |
Number of reflections | 8880 | 445 |
<I/σ(I)> | 30.48 | 2.66 |
Completeness [%] | 100.0 | 100 |
Redundancy | 5.7 | |
CC(1/2) | 0.980 | 0.780 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291 | 0.1 M MES pH 5.6, 5mM Magnesium chloride, 16% PEG MME 2000 |