8UJV
Crystal structure of human polymerase eta with incoming dCMPnPP nucleotide opposite urea lesion
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2022-10-13 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.97872 |
Spacegroup name | P 61 |
Unit cell lengths | 98.951, 98.951, 81.498 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.480 - 2.230 |
R-factor | 0.1685 |
Rwork | 0.166 |
R-free | 0.22240 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 0.943 |
Data reduction software | HKL-2000 (v721.2) |
Data scaling software | HKL-2000 (v721.2) |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1-4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.270 |
High resolution limit [Å] | 2.230 | 2.230 |
Rmerge | 0.070 | 0.400 |
Rpim | 0.040 | 0.200 |
Number of reflections | 22232 | 1086 |
<I/σ(I)> | 23.6 | 3.24 |
Completeness [%] | 100.0 | 100 |
Redundancy | 4.1 | 4 |
CC(1/2) | 0.994 | 0.810 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 0.1 M MES pH 5.5, 5mM magnesium chloride, 18% PEG 2000 MME |