8UHS

anti-Phosphohistidine Fab hSC44.ck.20.elbow bound to phosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-01
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.97741
Spacegroup name	P 43 21 2
Unit cell lengths	72.326, 72.326, 205.930
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.790 - 2.400
R-factor	0.2534
Rwork	0.252
R-free	0.28630
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.535
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.790	2.440
High resolution limit [Å]	2.400	2.400
Rmerge	0.071	0.761
Rmeas	0.075	0.830
Rpim	0.023	0.322
Number of reflections	22383	1088
<I/σ(I)>	42	2.48
Completeness [%]	100.0	99.7
Redundancy	9.8	6.2
CC(1/2)	1.000	0.805

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1M Hepes, pH 7.5, 20% PEG4000, 10% 2-propanol