8UHN

anti-Phosphohistidine Fab hSC44.ck.20.N32F with 3pHis peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-24
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.97741
Spacegroup name	P 21 21 21
Unit cell lengths	71.280, 74.033, 239.278
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.190 - 2.090
R-factor	0.2188
Rwork	0.217
R-free	0.24460
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.518
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.190	2.140
High resolution limit [Å]	2.090	2.090
Rmerge	0.101	1.300
Rmeas	0.110	1.440
Rpim	0.044	0.611
Number of reflections	71747	3417
<I/σ(I)>	16	1.2
Completeness [%]	95.2	92.7
Redundancy	5.8	5.2
CC(1/2)	0.993	0.400

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.08M sodium cacodylate, pH 6.5, 0.16M calcium acetate, 20% glycerol, 14.4% PEG-8000