8UH7

Structure of T4 Bacteriophage clamp loader bound to the T4 clamp, primer-template DNA, and ATP analog

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2008-11-25
Detector	ADSC QUANTUM 315
Wavelength(s)	0.973900
Spacegroup name	P 1 21 1
Unit cell lengths	93.351, 118.325, 132.882
Unit cell angles	90.00, 102.05, 90.00

Refinement procedure

Resolution	49.649 - 2.628
R-factor	0.1952
Rwork	0.193
R-free	0.24450
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.003
RMSD bond angle	0.527
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.649	2.922
High resolution limit [Å]	2.628	2.628
Rmerge	0.325
Rmeas	0.355
Rpim	0.141	0.865
Number of reflections	55509	2775
<I/σ(I)>	6.9
Completeness [%]	92.9
Redundancy	6.3
CC(1/2)	0.978	0.306

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	295	20% PEG4k, 0.1M Tris pH7.5, 10mM MgCl2, 20mM NaCl