8UFJ

Structure of M. mazei GS(R167L-A168G) apo form

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	I 2 2 2
Unit cell lengths	137.264, 167.546, 176.922
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.520 - 2.450
R-factor	0.1909
Rwork	0.190
R-free	0.22150
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.630
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.520	2.510
High resolution limit [Å]	2.450	2.450
Rpim	0.075	0.880
Number of reflections	67432	1717
<I/σ(I)>	11.1	0.7
Completeness [%]	90.3
Redundancy	5.7
CC(1/2)	0.999	0.257

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	35% Peg 200, 0.1 M Na Citrate pH 5.5