8UF4

Crystal structure of wildtype dystroglycan proteolytic domain (juxtamembrane domain)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-17
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979180
Spacegroup name	P 21 21 21
Unit cell lengths	44.945, 86.577, 128.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	51.430 - 2.430
R-factor	0.2272
Rwork	0.225
R-free	0.27450
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.003
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.430	2.519
High resolution limit [Å]	2.430	2.432
Rmerge	0.097	1.799
Rmeas	0.104	1.952
Rpim	0.039	0.741
Number of reflections	19288	12051
<I/σ(I)>	10.62	1.14
Completeness [%]	98.9	94.33
Redundancy	7.2	6.6
CC(1/2)	0.998	0.656

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.3	295	0.1 M Tris, 0.2 M lithium sulfate, 20% PEG 4000