8UEL

Crystal structure of enolase from Litopenaeus vannamei

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-01-17
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	1.276911
Spacegroup name	P 21 21 21
Unit cell lengths	79.613, 86.571, 155.881
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.680 - 2.490
R-factor	0.1712
Rwork	0.168
R-free	0.22440
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.925
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	MOLREP
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	10.000	2.540
High resolution limit [Å]	2.490	6.760	2.500
Rmerge	0.180	0.086	0.641
Rmeas	0.196	0.094	0.702
Rpim	0.077	0.039	0.285
Number of reflections	38505	2105	1871
<I/σ(I)>	4
Completeness [%]	100.0	100	99.9
Redundancy	6.4	5.8	5.9
CC(1/2)	0.982	0.992	0.868

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	(NH4)2SO4, MES pH 6.5, PEG 5000 monomethyl ether