8UE9

Structure of TREK-1CG*:CAT335

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9779
Spacegroup name	P 21 21 21
Unit cell lengths	67.069, 119.776, 129.708
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.620 - 3.000
R-factor	0.2696
Rwork	0.268
R-free	0.30600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.566
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1-4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.620	3.107
High resolution limit [Å]	3.000	3.000
Number of reflections	21576	2112
<I/σ(I)>	6.8
Completeness [%]	99.8
Redundancy	19
CC(1/2)	0.993	0.207

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		277	20-25% PEG4000, 200 mM KCl, 100mM HEPES pH 7.0-7.5, 1mM CdCl2