8UDU
The X-RAY co-crystal structure of human FGFR3 and Compound 17
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-07-27 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.700, 69.314, 78.473 |
| Unit cell angles | 90.00, 90.96, 90.00 |
Refinement procedure
| Resolution | 34.140 - 1.737 |
| R-factor | 0.2311 |
| Rwork | 0.230 |
| R-free | 0.25580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.780 |
| Data reduction software | autoPROC (1.1.7) |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.140 | 1.800 |
| High resolution limit [Å] | 1.737 | 1.737 |
| Rmerge | 0.040 | 0.910 |
| Rmeas | 0.040 | 0.950 |
| Rpim | 0.010 | 0.260 |
| Number of reflections | 61639 | 6122 |
| <I/σ(I)> | 26.9 | 2.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14 | 13.5 |
| CC(1/2) | 1.000 | 0.900 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.10 M Magnesium Chloride, 0.10 M TRIS/HCl pH 8.10, 0.20 M Sodium Chloride, 20.00 % (w/v) PEG 3350 |
