8UDN

A Stable Heterotrimeric Foldon with Minimum Mutation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.6199
Spacegroup name	P 43
Unit cell lengths	32.854, 32.854, 75.230
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.200 - 0.970
R-factor	0.1459
Rwork	0.145
R-free	0.15750
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.093
Data reduction software	XDS (Jan 10, 2022 BUILT=20220220)
Data scaling software	XDS (Jan 10, 2022 BUILT=20220220)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.110	30.110	0.990
High resolution limit [Å]	0.970	4.330	0.970
Rmerge	0.058	0.024	1.274
Rmeas	0.060	0.025	1.320
Number of reflections	46727	556	3444
<I/σ(I)>	17.4
Completeness [%]	99.0	98.9	98.3
Redundancy	14
CC(1/2)	1.000	0.999	0.759

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	MPD