8UDB

Crystal Structure of Mu class GST from TuGSTm12 (Tetur05g05300) from Tetranychus urticae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-08-03
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.000
Spacegroup name	P 31 2 1
Unit cell lengths	60.464, 60.464, 234.998
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.197 - 2.750
Rwork	0.215
R-free	0.26130
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.236
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.800
High resolution limit [Å]	2.750	2.750
Rmeas	0.143	0.782
Rpim	0.046	0.273
Number of reflections	13765	661
<I/σ(I)>	18	2.5
Completeness [%]	99.7	100
Redundancy	9.5	7.8
CC(1/2)	0.989	0.730

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M ammonium acetate, 0.1 M HEPES pH 7.5 and 25% w/v polyethylene glycol 3,350