8UD0
Sterile Alpha Motif (SAM) domain from Tric1 from Arabidopsis thaliana - G241E mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-06-06 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.953732 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.579, 58.537, 61.949 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.550 - 1.890 |
R-factor | 0.2017 |
Rwork | 0.200 |
R-free | 0.23680 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.730 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.550 | 1.960 |
High resolution limit [Å] | 1.890 | 1.890 |
Rmerge | 0.090 | 1.500 |
Rpim | 0.030 | 0.420 |
Number of reflections | 14037 | 1355 |
<I/σ(I)> | 18.9 | 2.41 |
Completeness [%] | 99.6 | 96.1 |
Redundancy | 13.1 | 13 |
CC(1/2) | 0.999 | 0.077 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.2 | 293.15 | 1.8-2.3 M ammonium sulphate, 0.1 M Tris |