8UCZ

Sterile Alpha Motif (SAM) domain from Tric1 from Arabidopsis thaliana - D235A mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-11-16
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.953700
Spacegroup name	P 21 21 21
Unit cell lengths	28.676, 92.587, 94.109
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.050 - 2.070
R-factor	0.249
Rwork	0.247
R-free	0.27660
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.833
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.050	2.140
High resolution limit [Å]	2.070	2.070
Rmerge	0.140	0.720
Rpim	0.030	0.019
Number of reflections	15930	1505
<I/σ(I)>	19.5	4
Completeness [%]	97.3	97.7
Redundancy	13.9	13.9
CC(1/2)	1.000	0.910

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293.15	1.5 M ammonium sulphate, 0.1 M Tris