8UBW

Choline-bound FLVCR1

EM specimen

Vitrification	Yes
Instrument	FEI VITROBOT MARK IV

EM Data Collection

Microscope	FEI TITAN KRIOS
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	300
Energy Filter	TFS Selectris X
Detector	FEI FALCON IV (4k x 4k)

3D Reconstruction

Method	SINGLE PARTICLE
Resolution (Å)	2.59
Number of Particle	2712047
Software	cryoSPARC,Leginon
Symmetry	C1
CTF correction	PHASE FLIPPING AND AMPLITUDE CORRECTION