8UB7
Diversity-generating retroelement (DGR) ribonucleoprotein reverse transcriptase - Active state (N-occupied)
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.2 |
| Software | RELION,Coot,cryoSPARC,CCP4 package,Gautomatch,Topaz,SerialEM,Gctf,CTFFIND |
| CTF correction | PHASE FLIPPING ONLY |
