8UB5

The Apo NanH structure from Clostridium perfringens

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-002
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-22
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.54
Spacegroup name	C 1 2 1
Unit cell lengths	93.436, 64.492, 65.747
Unit cell angles	90.00, 102.71, 90.00

Refinement procedure

Resolution	29.450 - 1.800
R-factor	0.1653
Rwork	0.164
R-free	0.19680
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.078
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.850
High resolution limit [Å]	1.800	1.800
Number of reflections	35412	1757
<I/σ(I)>	14.9
Completeness [%]	99.9
Redundancy	4
CC(1/2)	0.991	0.704

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1M HEPES:NaOH pH 7.5, 20% PEG 8000