8UB5
The Apo NanH structure from Clostridium perfringens
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-002 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-01-22 |
Detector | DECTRIS PILATUS 200K |
Wavelength(s) | 1.54 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 93.436, 64.492, 65.747 |
Unit cell angles | 90.00, 102.71, 90.00 |
Refinement procedure
Resolution | 29.450 - 1.800 |
R-factor | 0.1653 |
Rwork | 0.164 |
R-free | 0.19680 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.078 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.850 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 35412 | 1757 |
<I/σ(I)> | 14.9 | |
Completeness [%] | 99.9 | |
Redundancy | 4 | |
CC(1/2) | 0.991 | 0.704 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1M HEPES:NaOH pH 7.5, 20% PEG 8000 |