8UAV
Cryo-EM Structure of Brucella Abortus Lumazine Synthase (BLS) Engineered with Shiga Toxin I subunit B (Stx1B)
EM specimen
| Vitrification | Yes |
| Instrument | LEICA EM GP |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Energy Filter | GIF Bioquantum |
| Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2.37 |
| Number of Particle | 1773142 |
| Software | cryoSPARC,PHENIX,Topaz,EPU,Coot,UCSF ChimeraX |
| Symmetry | D5 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
