8UAG

Sucrose-phosphate synthase-like protein from Leishmania major

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-23
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.954
Spacegroup name	P 21 21 21
Unit cell lengths	40.231, 59.689, 177.638
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.410 - 2.290
R-factor	0.1601
Rwork	0.156
R-free	0.23770
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.103
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.410	2.370
High resolution limit [Å]	2.290	2.290
Rmerge	0.081	0.244
Rmeas	0.085	0.256
Rpim	0.026	0.079
Total number of observations	206017	18314
Number of reflections	19918	1829
<I/σ(I)>	19.6	8.5
Completeness [%]	99.4
Redundancy	10.3	10
CC(1/2)	0.999	0.980

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	22.5% v/v PEG Smear High, 10% v/v Ethylene Glycol, 0.1M MIB 4 pH