8U8M
X-ray crystal structure of TEBP-1 MCD2 homodimer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2022-06-03 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.97872 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 76.842, 59.965, 151.966 |
Unit cell angles | 90.00, 101.82, 90.00 |
Refinement procedure
Resolution | 49.580 - 2.700 |
R-factor | 0.234 |
Rwork | 0.232 |
R-free | 0.26520 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.511 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 148.740 | 2.830 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.069 | 0.560 |
Number of reflections | 35989 | 2686 |
<I/σ(I)> | 9.1 | |
Completeness [%] | 100.0 | |
Redundancy | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | TEBP-1 MCD2 + 1 M succinic acid, 9 mM cobalt chloride, 100 mM HEPES, pH 7.5, 16 degrees C, hanging drop, cryoprotectant: 1.2 M succinic acid, 7 mM cobalt chloride, 100 mM HEPES, pH 7.2 |