8U8C
Crystal structure of the TREX-2 complex in complex with the N-terminal motif of Sub2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-01 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.0081 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.861, 87.001, 169.802 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.360 - 2.400 |
| R-factor | 0.193 |
| Rwork | 0.191 |
| R-free | 0.23050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.464 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.440 |
| High resolution limit [Å] | 2.380 | 2.400 |
| Rpim | 0.044 | 0.218 |
| Number of reflections | 73285 | 2178 |
| <I/σ(I)> | 17.4 | |
| Completeness [%] | 94.8 | |
| Redundancy | 8.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M MES, pH 6.5, and 20% PEG3350 |
