8U7F
Crystal structure of CIB_12 beta-galactosidase from Cuniculiplasma divulgatum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2019-10-04 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 193.215, 51.805, 128.794 |
Unit cell angles | 90.00, 108.34, 90.00 |
Refinement procedure
Resolution | 24.930 - 2.550 |
R-factor | 0.2217 |
Rwork | 0.220 |
R-free | 0.25970 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.553 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.20_4459) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.590 |
High resolution limit [Å] | 2.550 | 2.550 |
Rmerge | 0.093 | 0.581 |
Rpim | 0.047 | 0.289 |
Number of reflections | 38156 | 1629 |
<I/σ(I)> | 18.34 | 1.54 |
Completeness [%] | 95.2 | 81.5 |
Redundancy | 4.9 | 4.7 |
CC(1/2) | 0.886 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | PEG 3350 25%, 0.2 M sodium tartrate, 0.1 M Tris pH 8.5, 0.3 M NDSB 201, 1% 1-butyl-2,3-dimethylimidazolium tetrafluoroborate |