8U53
Mechanically activated ion channel OSCA3.1 in nanodiscs
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 2.6 |
Number of Particle | 1913316 |
Software | cryoSPARC,RELION,Coot,PHENIX,Rosetta,Leginon,Gctf,UCSF Chimera |
Symmetry | C2 |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |