8U21

A Highly Stable Variant of Corynactis Californica Green Fluorescent Protein, ccGFP E6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-16
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97741
Spacegroup name	C 2 2 21
Unit cell lengths	87.647, 146.083, 50.758
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.580 - 1.960
R-factor	0.1793
Rwork	0.177
R-free	0.21170
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.004
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.980
High resolution limit [Å]	1.950	5.290	1.950
Rmerge	0.078	0.042	0.560
Total number of observations	92961
Number of reflections	23695	1287	1172
<I/σ(I)>	9.4
Completeness [%]	99.7	99.2	100
Redundancy	3.9	3.7	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.25M (NH4)2SO4, 0.1M Sodium acetate pH 5.0, 30% PEG4K, 5% Glycerol