8U1J
N-Terminal domain of DNA-Damage Response Protein C (DdrC) from Deinococcus radiodurans - Crystal form xMJ7102
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-09 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97995 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 73.410, 73.410, 110.230 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.790 - 3.000 |
R-factor | 0.2268 |
Rwork | 0.225 |
R-free | 0.25730 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.020 |
RMSD bond angle | 1.662 |
Data reduction software | XDS (20190315) |
Data scaling software | XSCALE (20230630) |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.790 | 3.023 |
High resolution limit [Å] | 2.972 | 2.972 |
Rmerge | 0.060 | 0.462 |
Rmeas | 0.062 | 0.485 |
Rpim | 0.014 | 0.107 |
Number of reflections | 3335 | 164 |
<I/σ(I)> | 33.7 | 5.4 |
Completeness [%] | 100.0 | 100 |
Redundancy | 20.9 | 20.2 |
CC(1/2) | 0.998 | 0.956 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 3.0 ul of protein solution was mixed with 1.0 uL of crystallization solution and hung upside-down in a sealed chamber containing 1mL of well solution. During crystallization, an unknown protease contaminant in the protein solution cleaved FL DdrC between residues 98 and 99, allowing for crystallization of residues 1-98. Protein solution: 257uM DdrC, 800mM NaCl 20mM Tris, pH 8.0 5% (v/v) Glycerol Unknown protease contaminant Crystallization solution (Midas1 - MD1-59 condition #91): 15% (v/v) Sokolan-CP42 200mM Potassium citrate tribasic Well solution: 3.25M Ammonium sulfate |