8U16

The ternary complex structure of DDB1-CRBN-SALL4(ZF1,2)-short bound to Pomalidomide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2019-11-26
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	95.536, 151.986, 218.460
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.350 - 2.900
R-factor	0.2217
Rwork	0.219
R-free	0.27270
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.150
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	88.700	2.970
High resolution limit [Å]	2.900	2.900
Rmerge	0.077	8.198
Rmeas	0.609	8.365
Rpim	0.118	1.660
Total number of observations	1886081	113305
Number of reflections	71316	4497
<I/σ(I)>	5	0.6
Completeness [%]	100.0
Redundancy	26.4	25.2
CC(1/2)	0.995	0.432

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2M Sodium malonate, 20% PEG 3350