8U0Y
Bacterial protein cpx
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 6 2 2 |
| Unit cell lengths | 221.790, 221.790, 139.340 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 79.080 - 3.000 |
| R-factor | 0.2344 |
| Rwork | 0.233 |
| R-free | 0.25830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1eyx |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.422 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 96.040 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rpim | 0.114 | 0.484 |
| Number of reflections | 40827 | 5859 |
| <I/σ(I)> | 7.4 | 2.1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 8.4 | 7.9 |
| CC(1/2) | 0.965 | 0.407 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 0.1 M sodium acetate, pH 4.5, 1.2 M ammonium sulfate, 5% w/v D-sorbitol |
