8U0R

The crystal structure of protein A21, a component of the conserved poxvirus entry-fusion complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	RIGAKU MICROMAX-003
Temperature [K]	95
Detector technology	IMAGE PLATE
Collection date	2016-10-28
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54178
Spacegroup name	P 1 21 1
Unit cell lengths	46.860, 70.060, 78.950
Unit cell angles	90.00, 95.29, 90.00

Refinement procedure

Resolution	39.310 - 2.300
R-factor	0.2136
Rwork	0.211
R-free	0.23940
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.301
Data reduction software	CrystalClear
Data scaling software	CrystalClear
Phasing software	AutoSol
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.310	2.360
High resolution limit [Å]	2.300	2.300
Rmerge	0.101	0.333
Rmeas	0.118	0.399
Number of reflections	22602	1595
<I/σ(I)>	8.2	3.1
Completeness [%]	99.2	91
Redundancy	7.31	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	One mL of protein at a concentration of 10 mg/ml in i10 mM Tris-HCl, 50 mM NaCl, pH 8.0 was mixed with 1 mL of mother liquor containing 20% PEG 4000, 0.1 M Na-citrate, 10% ethanol, and 1-5% cocktail mixture of low molecular alcohols,