8U0G

Full-length dimer of DNA-Damage Response Protein C from Deinococcus radiodurans - Crystal form xMJ7124

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2022-09-23
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.541780
Spacegroup name	P 32 2 1
Unit cell lengths	111.039, 111.039, 101.493
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	69.810 - 4.280
R-factor	0.2567
Rwork	0.252
R-free	0.34570
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.978
Data reduction software	XDS (20220220)
Data scaling software	pointless (0.7.7)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.19.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	96.163	4.351
High resolution limit [Å]	4.277	4.277
Rmerge	0.179	0.937
Rpim	0.064	0.344
Number of reflections	5236	242
<I/σ(I)>	9.9	2.9
Completeness [%]	100.0	100
Redundancy	8.9	9.1
CC(1/2)	0.998	0.889

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1.5 ul of protein solution was mixed with 1.5 uL of crystallization solution and hung upside-down in a sealed chamber containing 1mL of well solution Protein solution: 140uM DdrC, 200mM Sodium sulfate, 1mM Magnesium chloride, 20mM Sodium citrate / Citric acid, pH 6.5 Crystallization solution (Wizard Classics 1 #22) 10% (v/v) 2-propanol 100mM Tris-base/HCl, pH 8.5 Well solution: 1.5M Ammonium sulfate