8U0B

Crystal structure of Lyssavirus rabies (Nishigahara strain) nucleoprotein in complex with phosphomimetic phosphoprotein S48E

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-08-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	41.894, 75.393, 154.985
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.890 - 2.100
R-factor	0.1844
Rwork	0.183
R-free	0.21480
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.671
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.880	2.160
High resolution limit [Å]	2.100	2.100
Rmerge	0.138	0.854
Number of reflections	138385	2335
<I/σ(I)>	5.9
Completeness [%]	99.9
Redundancy	4.7
CC(1/2)	0.989	0.849

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M lithium acetate trihydrate, 20% polyethylene glycol 3350