8U08

Crystal structure of 10E8-GT11 scaffold in complex with a human 10E8 inferred germline (10E8-iGL1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0332
Spacegroup name	P 21 21 21
Unit cell lengths	69.749, 73.905, 129.882
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.780 - 1.980
R-factor	0.1886
Rwork	0.186
R-free	0.23580
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.131
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.800	2.010
High resolution limit [Å]	1.980	1.980
Rpim	0.052	0.412
Number of reflections	43220	1662
<I/σ(I)>	13	1.2
Completeness [%]	92.1	70.8
Redundancy	7.9	5.6
CC(1/2)	0.850	0.430

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.095 M NaCitrate,19% 2-propanol, 5% glycerol, 19% PEG4000