8U01
Crystal Structure of the Glycoside Hydrolase Family 2 TIM Barrel-domain Containing Protein from Phocaeicola plebeius
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-11-09 |
| Detector | DECTRIS PILATUS3 X 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 41 3 2 |
| Unit cell lengths | 230.110, 230.110, 230.110 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 69.380 - 3.000 |
| R-factor | 0.1551 |
| Rwork | 0.153 |
| R-free | 0.18610 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.565 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.800 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.137 | 0.524 |
| Rmeas | 0.139 | 0.534 |
| Rpim | 0.027 | 0.105 |
| Number of reflections | 42107 | 2039 |
| <I/σ(I)> | 28.9 | 7.5 |
| Completeness [%] | 100.0 | 99.1 |
| Redundancy | 26 | 7.5 |
| CC(1/2) | 0.999 | 0.974 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.2 M sodium chloride, 0.1 M HEPES, pH 7.5, 30% v/v PEG400 |
