8TZW

Crystal structure of 10E8-GT4 scaffold in complex with a human 10E8 inferred germline (10E8-iGL1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-03-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 1 21 1
Unit cell lengths	71.487, 70.988, 73.224
Unit cell angles	90.00, 107.36, 90.00

Refinement procedure

Resolution	31.700 - 2.660
R-factor	0.2185
Rwork	0.216
R-free	0.25920
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.524
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.21rc1_5058)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.700	2.710
High resolution limit [Å]	2.660	2.660
Rpim	0.067	0.469
Number of reflections	19803	986
<I/σ(I)>	11.6	2.2
Completeness [%]	96.5	97.6
Redundancy	3.2	3.3
CC(1/2)	0.970	0.730

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	10% glycerol, 0.1 M HEPES pH 7.5 5% PEG 3000, 30% PEG 400